Fecha/hora: 20/05/2024 14:43:37

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	22/09/2023	
Fecha de la última actualización: 	22/09/2023	
Autoria: 	BERAIS-RUBIO, A.; MOREL-REVETRIA, M.; FILIPPI, C.V.; REYNO, R.; MONZA, J.	
Afiliación: 	ANDRÉS BERAIS-RUBIO, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay; MARÍA MOREL-REVETRIA, Laboratorio de Microbiología de Suelos (LMS), Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo PC 11.400, Uruguay; CARLA VALERIA FILIPPI, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay.	
Título: 	Ensifer meliloti elite strain U143 used as alfalfa inoculant in Uruguay: Characterization and draft genome sequence.	
Año de publicación: 	2023	
Fuente/Imprenta: 	The Microbe. 2023, Volume 1, article 100008. https://doi.org/10.1016/j.microb.2023.100008  -- OPEN ACCESS.	
ISSN: 	2950-1946	
DOI: 	10.1016/j.microb.2023.100008	
Idioma: 	Inglés	
Notas: 	Article history: Received 23 May 2023, Revised 14 August 2023, Accepted 12 September 2023, Available online 13 September 2023, Version of Record 19 September 2023. -- Correspondence: Jorge Monza, mail: jmonza@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). -- FUNDING: This work was financially supported by Instituto Nacional de
Investigación Agropecuaria (INIA) L-1, Grant Comisión Académica de Postgrado, Universidad de la República, Programa de Desarrollo de
Ciencias Básicas (PEDECIBA) and Sistema Nacional de Investigadores (SNI).	
Contenido: 	ABSTRACT.- Ensifer (syn. Sinorhizobium) meliloti U143 is an effective nitrogen-fixing strain isolated from Uruguayan soils. For decades, this strain has been used as an inoculant for different alfalfa cultivars. Here we report for the first time a characterization of the U143 elite strain that includes the preliminary genomic sequence, its annotation, and physiological parameters related to its symbiotic efficiency and nitrate respiration capacity. Through Illumina sequencing, the genome of the U143 strain was sequenced. The genome length was 6,801,966 bp, and it contained two megaplasmids, an average GC content of 62.15 %, and 6522 protein-coding sequences. In the symbiotic plasmid, we identified nap, nir, nor, and nos sequences that explain the ability of the U143 strain to respire NO3- in free-living and microaerobic conditions. Field assays performed in two locations for two years showed that alfalfa inoculated with the U143 strain produced 41 % more total shoot dry matter than the non-inoculated control, and between 61.3 % and 66.5 % of shoot N in alfalfa inoculated with strain U143 derived from nitrogen fixation. © 2023 The Authors. Published by Elsevier Ltd	
Palabras-Clave: 	Draft genome; Elite strain; Inoculant.	
Categoría del tema: 	A50 Investigación agraria	
URL: 	https://www.sciencedirect.com/science/article/pii/S2950194623000080/pdf	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (103660-1) - PXI - AP - DD - The Microbe/2023 -  -  - DIS